Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The human papillomavirus type 16 (HPV-16) status of 43 cervical biopsies, which had been characterized histologically as normal, various grades of cervical intraepithelial neoplasia (CIN) and invasive squamous cell carcinoma, was examined by using (i) a novel antibody against the HPV-16 E2 protein, (ii) sensitive HPV-16 DNA in situ hybridization and (iii) microdissection/PCR for the E2 ORF. The data indicate that E2 protein expression is highest in koilocytes in lower-grade CIN (I), but decreases with increasing grade, whereas the detection of HPV DNA is delayed until CIN I/II, rising to the highest levels in carcinoma cells. Co-localization of E2 with HPV-16 DNA-positive cells was most commonly observed in koilocytes in CIN II lesions. PCR analyses of microdissected epithelium from the same or serial sections indicated that E2 ORFs were retained in an intact form in a number of higher-grade CIN lesions and invasive carcinomas.

Original publication

DOI

10.1099/0022-1317-81-7-1825

Type

Journal article

Journal

J Gen Virol

Publication Date

07/2000

Volume

81

Pages

1825 - 1832

Keywords

Carcinoma, Squamous Cell, Cervical Intraepithelial Neoplasia, DNA, Viral, DNA-Binding Proteins, Female, Humans, In Situ Hybridization, Oncogene Proteins, Viral, Open Reading Frames, Papillomaviridae, Polymerase Chain Reaction, Uterine Cervical Neoplasms