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In an attempt to express the two distal genes of the Escherichia coli threonine operon, the majority of the first gene in the operon, thrA, was removed and a series of transcriptional fusions were constructed placing the thrB and thrC genes downstream of either the trp or hybrid tac promoter. Analysis of the proteins produced by cells containing these fusions revealed that although the distal gene, thrC, was efficiently expressed, the proximal gene, thrB, was not expressed at a detectable level. A translational fusion was constructed which fused the cat gene in phase to the last 800 base pairs of thrA followed by thrB and thrC. Cells containing this fusion produced high levels of both the thrB and thrC gene products, showing that translation of thrB requires translation through thrA; thus, thrA and thrB are translationally coupled. In addition, it was found that a sequence between 220 and 57 base pairs before the start of thrB was necessary to allow translational coupling to occur.

Original publication

DOI

10.1128/jb.171.6.3518-3522.1989

Type

Journal article

Journal

J Bacteriol

Publication Date

06/1989

Volume

171

Pages

3518 - 3522

Keywords

Carbon-Oxygen Lyases, Cloning, Molecular, Escherichia coli, Gene Expression Regulation, Genes, Bacterial, Homoserine Dehydrogenase, Lyases, Molecular Weight, Operon, Phosphotransferases, Phosphotransferases (Alcohol Group Acceptor), Protein Biosynthesis, Recombinant Fusion Proteins, Threonine