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CD22 is a B cell-specific transmembrane protein known to function as a negative regulator of B cell signaling. It has also been implicated in cell adhesion through recognition of alpha2,6-linked sialic acids on glycans of target cells. Previous studies showed that CD22-deficient mice had a strongly reduced population of mature recirculating B cells in the bone marrow despite normal B cell development. Using a soluble recombinant form of the receptor (CD22-Fc), we demonstrate here that sialylated ligands for CD22 are expressed on sinusoidal endothelial cells of murine bone marrow but not on endothelial cells in other tissues examined. Injection of CD22-Fc revealed that the CD22 ligands in the bone marrow were accessible to the circulation. Treatment of mice with either CD22-Fc or affinity-purified anti-CD22 antibody led to an approximately 50% reduction in mature recirculating B cells in the bone marrow without affecting numbers in the spleen. Finally, consistent with the notion that CD22 is a homing receptor, we show that compared with wild-type mice, CD22-deficient animals have a lower number of immunoglobulin M-secreting plasma cells in the bone marrow.

Original publication

DOI

10.1084/jem.189.9.1513

Type

Journal article

Journal

J Exp Med

Publication Date

03/05/1999

Volume

189

Pages

1513 - 1518

Keywords

Animals, Antigens, CD, Antigens, Differentiation, B-Lymphocyte, B-Lymphocytes, Bone Marrow, CHO Cells, Cell Adhesion Molecules, Cell Movement, Cricetinae, Endothelium, Lectins, Ligands, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, N-Acetylneuraminic Acid, Sialic Acid Binding Ig-like Lectin 2