Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

Original publication

DOI

10.21769/BioProtoc.2645

Type

Journal article

Journal

Bio Protoc

Publication Date

05/12/2017

Volume

7

Keywords

Capture-C, Chromatin architecture, Chromosome conformation capture (3C), Low input, Nuclear organization, Small cell numbers