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Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

Original publication

DOI

10.21769/BioProtoc.2645

Type

Journal article

Journal

Bio Protoc

Publication Date

05/12/2017

Volume

7

Keywords

Capture-C, Chromatin architecture, Chromosome conformation capture (3C), Low input, Nuclear organization, Small cell numbers