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Phosphatidylinositol (PI) 3-kinase is a heterodimeric enzyme comprising a 110-kDa catalytic subunit and an 85-kDa regulatory subunit that binds to tyrosine phosphopeptide sites linked directly or indirectly to receptors serving diverse signal functions. Knowledge of the structure and function of PI 3-kinase was greatly advanced by the purification, cDNA cloning, and subsequent expression of the bovine enzyme. Here the cloning of the cDNA for the human p110 alpha subunit of PI 3-kinase (PIK3CA), encoding a protein 99% identical to the bovine p110, and of its gene in YAC is described. The chromosomal localization of the gene for PIK3CA is shown to be at 3q21-qter as determined using somatic cell hybrids. In situ hybridization performed using Alu-PCR from the YAC DNA located the gene in 3q26.3.

Type

Journal article

Journal

Genomics

Publication Date

12/1994

Volume

24

Pages

472 - 477

Keywords

Animals, Base Sequence, Cattle, Chromosome Mapping, Chromosomes, Artificial, Yeast, Chromosomes, Human, Pair 3, Cloning, Molecular, Cricetinae, DNA, Complementary, Humans, Hybrid Cells, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phosphatidylinositol 3-Kinases, Phosphotransferases (Alcohol Group Acceptor), Polymerase Chain Reaction, Protein Conformation