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The relationship of seven monoclonal antibodies, putatively to the Bo5 (CD5) antigen, was tested. Five of the mAbs were confirmed to be directed against the Bo5 antigen. Three mAbs, CC29, BLT-1 and 8C11, effectively blocked binding to bovine PBM of mAb CC17, previously reported to be directed against this antigen. MAb 8-3F4 also blocked binding of mAb CC17, but less effectively than the others. MAbs IL-A67 and 79-5 did not inhibit binding of mAb CC17 because of antibody allelic specificity or technical reasons.

Type

Journal article

Journal

Vet Immunol Immunopathol

Publication Date

01/1991

Volume

27

Pages

147 - 151

Keywords

Animals, Antibodies, Monoclonal, Antigens, Differentiation, Binding, Competitive, CD5 Antigens, Cattle, Flow Cytometry, Leukocytes, Mononuclear