Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Flow cytometry and two-colour immunofluorescence were used to detect cytoplasmic bovine viral diarrhoea virus (BVDV) antigen in leukocytes from viraemic cattle. Monoclonal antibody to the p80 protein of BVDV, a non-structural viral antigen, was used to identify the subpopulations of leukocytes in which viral protein synthesis had occurred. Viral antigen was detected in 23% of peripheral blood mononuclear cells. Monocytes were found to have the highest frequency of infection (35%). A higher proportion of CD2+ T cells (23%) were infected, compared with B cells (11%) or WC1+ gamma delta T cells (11%). No significant differences in percentages of different leukocyte subpopulations in blood were detected in persistently viraemic animals compared with controls.

Original publication

DOI

10.1099/0022-1317-75-5-1189

Type

Journal article

Journal

J Gen Virol

Publication Date

05/1994

Volume

75 ( Pt 5)

Pages

1189 - 1194

Keywords

Animals, Antibodies, Monoclonal, Antibodies, Viral, Antigens, Viral, Bovine Virus Diarrhea-Mucosal Disease, Cattle, Flow Cytometry, Leukocytes, Monocytes, Viral Nonstructural Proteins, Viremia