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Six monoclonal antibodies (mAbs) that clustered together in a statistical analysis of data submitted to the Third Workshop on Ruminant Leucocyte Antigens and mAb CC-G33 were tested for ability to stain COS-7 cells transfected with cDNA encoding human CD14. Only mAb CC-G33 recognised the human molecule. The six mAbs were compared with mAb CC-G33 by flow cytometry and three were shown to be directed against bovine CD14.

Type

Journal article

Journal

Vet Immunol Immunopathol

Publication Date

08/1996

Volume

52

Pages

323 - 328

Keywords

Animals, Antibodies, Monoclonal, COS Cells, Cattle, Flow Cytometry, Humans, Lipopolysaccharide Receptors, Transfection