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Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able to accurately discriminate Mycobacterium bovis-infected from BCG-vaccinated animals. Herein, we demonstrate that the detection of secreted IFN-gamma following short term culture (4h) of whole blood with purified protein derived from M. bovis (PPD-B) allows such discrimination. This reflects, in part, the differential kinetics of IFN-gamma secretion in infected compared to vaccinated cattle. This is the first study to demonstrate that accurate, rapid distinction of BCG-vaccinated from M. bovis-infected cattle can be achieved in a short time period without the need for production of M. bovis-specific antigens, complex antigen mixtures or extensive laboratory procedures. We were also able to detect PPD-specific IFN-gamma release during short term culture of blood from a number of humans with active TB indicating that this test may have wider application and is potentially useful for the rapid diagnosis of disease in humans.

Original publication

DOI

10.1016/j.vaccine.2008.07.059

Type

Journal article

Journal

Vaccine

Publication Date

09/10/2008

Volume

26

Pages

5470 - 5476

Keywords

Animals, Antigens, Bacterial, BCG Vaccine, CD4-Positive T-Lymphocytes, Cattle, Cytokines, Flow Cytometry, Humans, Interferon-gamma, Kinetics, Mycobacterium bovis, Tuberculosis, Bovine, Tuberculosis, Pulmonary, Vaccination