Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorporation throughout the lagging strand.

Original publication

DOI

10.1016/j.jmb.2010.02.029

Type

Journal article

Journal

J Mol Biol

Publication Date

16/04/2010

Volume

397

Pages

1144 - 1155

Keywords

Animals, DNA, DNA Replication, DNA, Mitochondrial, Humans, Mammals, Nucleic Acid Conformation, Nucleic Acid Hybridization, RNA