Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Chromosome conformation capture (3C) methods are central to understanding the link between nuclear structure and function, and the physical interactions between distal regulatory elements and promoters. However, no one method is appropriate to address all biological questions, as each variant differs markedly in resolution, reproducibility, throughput and biases. A thorough appreciation of the strengths and weaknesses of each technique is critical when choosing the correct method for a specific application or for gauging how best to interpret different sources of data. In addition, the analysis method must be carefully considered, as this choice can profoundly affect the output. In this Review, we describe and compare the different available 3C-based approaches, with a focus on the analysis of mammalian genomes.

Original publication

DOI

10.1038/nmeth.4146

Type

Journal article

Journal

Nat Methods

Publication Date

31/01/2017

Volume

14

Pages

125 - 134

Keywords

Animals, Chromatin, Chromosome Mapping, Chromosomes, Gene Library, Genetic Techniques, High-Throughput Screening Assays, Humans, In Situ Hybridization, Fluorescence, K562 Cells, Mice, Polymerase Chain Reaction, SOXB1 Transcription Factors, alpha-Globins