Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In addition to direct assessment of high energy phosphorus containing metabolite content within tissues, phosphorus magnetic resonance spectroscopy ((31)P-MRS) provides options to measure phospholipid metabolites and cellular pH, as well as the kinetics of chemical reactions of energy metabolism in vivo. Even though the great potential of (31)P-MR was recognized over 30 years ago, modern MR systems, as well as new, dedicated hardware and measurement techniques provide further opportunities for research of human biochemistry. This paper presents a methodological overview of the (31)P-MR techniques that can be used for basic, physiological, or clinical research of human skeletal muscle and liver in vivo. Practical issues of (31)P-MRS experiments and examples of potential applications are also provided. As signal localization is essential for liver (31)P-MRS and is important for dynamic muscle examinations as well, typical localization strategies for (31)P-MR are also described.

Original publication

DOI

10.1016/j.ab.2017.01.018

Type

Journal article

Journal

Anal Biochem

Publication Date

15/07/2017

Volume

529

Pages

193 - 215

Keywords

Energy metabolism, Exercise-recovery, Liver, Phosphorus magnetic resonance spectroscopy, Saturation transfer, Skeletal muscle, Animals, Energy Metabolism, Humans, Liver, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Models, Biological, Muscle, Skeletal, Phosphorus Isotopes