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The aim of this study was to introduce a two-dimensional chemical shift imaging (2D CSI) sequence, with simultaneous acquisition of free induction decay (FID) and long TEs, for the detection and quantification of intramyocellular lipids (IMCLs) in the calf at 7 T. The feasibility of the new 2D CSI sequence, which acquires FID (acquisition delay, 1.3 ms) and an echo (long TE) in one measurement, was evaluated in phantoms and volunteers (n = 5): TR/TE*/TE = 800/1.3/156 ms; 48 × 48 matrix; field of view, 200 × 200 × 20 mm(3) ; Hamming filter; no water suppression; measurement time, 22 min 2 s. The IMCL concentration and subcutaneous lipid contamination were assessed. Spectra in the tibialis anterior (TA), gastrocnemius (GM) and soleus (SOL) muscles were analyzed. The water signal from the FID acquisition was used as an internal concentration reference. In the spectra from subcutaneous adipose tissue (SUB) and bone marrow (BM), an unsaturation index (UI) of the vinyl-H (5.3 ppm) to methyl-CH3 ratio, and a polyunsaturation index (pUI) of the diallylic-H (2.77 ppm) to -CH3 ratio, were calculated. Long-TE spectra from muscles showed a simplified spectral pattern with well-separated IMCL for several muscle groups in the same scan. The IMCL to water ratio was largest in SOL (0.66% ± 0.23%), and lower in GM (0.37% ± 0.14%) and TA (0.36% ± 0.12%). UI and pUI for SUB were 0.65 ± 0.06 and 0.18 ± 0.04, respectively, and for BM were 0.60 ± 0.16 and 0.18 ± 0.08, respectively. The new sequence, with the proposed name 'free induction decay echo spectroscopic imaging' (FIDESI), provides information on both specific lipid resonances and water signal from different tissues in the calf, with high spectral and spatial resolution, as well as minimal voxel bleeding and subcutaneous lipid contamination, in clinically acceptable measurement times.

Original publication




Journal article


NMR Biomed

Publication Date





980 - 987


1H chemical shift imaging (1H CSI), 7 T, calf, free induction decay (FID), intramyocellular lipid (IMCL), long TE, Adult, Creatine, Female, Humans, Leg, Lipids, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Male, Muscles, Phantoms, Imaging, Signal Processing, Computer-Assisted, Time Factors, Young Adult