Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1(+/-) embryos are heterogeneous and include CD45(+) cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of embryonic HSCs and contribute new insight into their cellular origin.

Type

Journal article

Journal

Immunity

Publication Date

05/2002

Volume

16

Pages

661 - 672

Keywords

Animals, Biomarkers, Cell Lineage, Core Binding Factor Alpha 2 Subunit, DNA-Binding Proteins, Embryo, Mammalian, Endothelium, Vascular, Gene Dosage, Gestational Age, Hematopoietic Stem Cells, Immunophenotyping, Leukocyte Common Antigens, Mesoderm, Mice, Mice, Inbred C57BL, Models, Biological, Proto-Oncogene Proteins, Transcription Factors