Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Biological effects of tumor necrosis factor alpha (TNF-alpha) are mediated through two cell surface receptors, the 55-kDa TNF receptor and the 75-kDa TNF receptor. The present study investigated the relative roles of the two TNF receptors in normal hematopoiesis. Using agonists (antibodies) specific for the 55- and 75-kDa TNF receptors, we demonstrate differential roles of the two TNF receptors in hematopoiesis in that only the 55-kDa TNF receptor mediates antiproliferative effects of TNF-alpha on mature Lin- hematopoietic progenitor cells responding to granulocyte colony-stimulating factor or interleukin 3 alone. In contrast, the 75-kDa TNF receptor is essential in mediating inhibition of primitive Lin-Sca-1+ high-proliferative-potential colony-forming cells and inhibition of the total number of proliferative clones of individually cultured Lin-Sca-1+Rh123lo and Lin-Sca-1+Rh123hi cells.

Type

Journal article

Journal

Proc Natl Acad Sci U S A

Publication Date

25/10/1994

Volume

91

Pages

10695 - 10699

Keywords

Animals, Bone Marrow Cells, Cell Differentiation, Cell Division, Cells, Cultured, Colony-Forming Units Assay, Granulocyte Colony-Stimulating Factor, Granulocyte-Macrophage Colony-Stimulating Factor, Hematopoiesis, Hematopoietic Stem Cells, Humans, Macrophage Colony-Stimulating Factor, Mice, Mice, Inbred C57BL, Molecular Weight, Radioligand Assay, Receptors, Tumor Necrosis Factor, Recombinant Proteins, Tumor Necrosis Factor-alpha