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Interleukin-7 (IL-7) has been shown to be a critical factor in murine lymphoid development. It stimulates pre-B cells to divide in the absence of stroma cells and it is an important growth regulator of immature and mature T cells. IL-7 has been shown to synergize with stem cell factor (SCF) to provide a potent growth stimulus for pre-B cells. However, the combined effects of IL-7 and SCF on murine primitive hematopoietic cells in vitro have not been established. In the present study, the effects of recombinant rat (rr) SCF and recombinant human (rh) IL-7 on primitive murine bone marrow progenitors (Lin-Sca1+) were investigated in single-cell cloning experiments. rhIL-7 alone had no proliferative effect on Lin-Sca1+ cells, but in a dose-dependent manner directly enhanced rrSCF-induced colony formation, with an average increase in colony numbers of 2.7-fold. Interestingly, the cells formed in response to SCF and IL-7 were predominantly mature granulocytes. Thus, SCF and IL-7 synergize to stimulate early myelopoiesis in vitro.

Type

Journal article

Journal

Blood

Publication Date

01/09/1994

Volume

84

Pages

1450 - 1456

Keywords

Animals, Bone Marrow, Bone Marrow Cells, Cell Adhesion Molecules, Cell Differentiation, Cell Division, Cells, Cultured, Culture Techniques, Drug Synergism, Flow Cytometry, Granulocytes, Hematopoietic Cell Growth Factors, Hematopoietic Stem Cells, Humans, Interleukin-7, Mice, Mice, Inbred C57BL, Rats, Recombinant Proteins, Stem Cell Factor