Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Preincubation of rat pancreatic islets with AICA riboside (0.1 to 1.0mM) caused a concentration-related stimulation of both 45Ca net uptake and insulin release evoked by 8.3 mM D-glucose, but failed to affect the conversion of D-[5-3H]glucose to 3HOH, the generation of 14CO2 and 14C-labelled amino acids or acidic metabolites from D-[U-14C]glucose, and the islet content in ATP, ADP or AMP. The secretory response to AICA riboside was not suppressed in islets preincubated with methotrexate. AICA riboside caused a progressive decrease in 86Rb outflow from prelabelled islets perifused at 2.8 or 6.0mM D-glucose. This effect faded out at a higher concentration of D-glucose (16.7 mM), in which case AICA riboside nevertheless provoked a delayed, progressive and not rapidly reversible enhancement of insulin output. At concentrations up to 0.4 mM, ZTP only exerted a modest effect on the activity of KATP-channels in inside-out patches of dispersed mouse islet cells. These findings raise the question whether the insulinotropic action of AICA riboside may be attributable to the sequential generation of ZMP, ZDP and ZTP from the nucleoside.

Type

Journal article

Journal

Diabetes Res

Publication Date

1994

Volume

25

Pages

25 - 37

Keywords

Adenine Nucleotides, Amino Acids, Aminoimidazole Carboxamide, Animals, Calcium, Egtazic Acid, Female, Glucose, Glycolysis, In Vitro Techniques, Insulin, Islets of Langerhans, Kinetics, Membrane Potentials, Methotrexate, Mice, Mice, Inbred Strains, Patch-Clamp Techniques, Rats, Rats, Wistar, Ribonucleosides, Rubidium, Time Factors