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Toll-like receptor 7 (TLR7) triggers antiviral immune responses by recognizing viral single-stranded RNA in endosomes, but the biosynthetic pathway of human TLR7 (hTLR7) remains unclear. Here, we show that hTLR7 is proteolytically processed and that the C-terminal fragment selectively accumulates in endocytic compartments. hTLR7 processing occurred at neutral pH and was dependent on furin-like proprotein convertases (PCs). Furthermore, TLR7 processing was required for its functional response to TLR7 agonists such as R837 or influenza virus. Notably, proinflammatory and differentiation stimuli increased the expression of furin-like PCs in immune cells, suggesting a positive feedback mechanism for TLR7 processing during infection. Because self-RNA can under certain conditions activate TLR7 and trigger autoimmunity, our results identify furin-like PCs as a possible target to attenuate TLR7-dependent autoimmunity and other immune pathologies.

Original publication

DOI

10.1016/j.immuni.2013.09.004

Type

Journal article

Journal

Immunity

Publication Date

10/2013

Volume

39

Pages

711 - 721

Addresses

MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DU, UK.

Keywords

Cell Line, Endosomes, Macrophages, Humans, Orthomyxoviridae, Lentivirus, Quinolines, Furin, Proprotein Convertases, Signal Transduction, Autoimmunity, Gene Expression Regulation, Protein Processing, Post-Translational, Amino Acid Sequence, Protein Structure, Tertiary, Genetic Vectors, Molecular Sequence Data, Toll-Like Receptor 7, Feedback, Physiological