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BACKGROUND AND OBJECTIVES: Autophagy is a self-degradation mechanism induced under stress conditions in all eukaryotic cells. Its activity in human lymphomas has not been studied as yet. METHODS: In this study, the autophagic activity of lymphoid cells was investigated in follicular lymphomas (FL; 48 cases), diffuse large B-cell lymphomas (DLBCL; 78 cases), and in reactive follicular hyperplasias (41 cases), using the light chain 3A (LC3A) antibody and a standard immunohistochemical technique. RESULTS: In all cases, the pattern of LC3A reactivity was uniformly diffuse cytoplasmic, but expressed more frequently in FLs (68.8%) than in DLBCLs (41%) (p=0.02), and much more commonly in DLBCLs than in reactive lymph nodes (24.3%) (p<0.006). Interestingly, FLs expressing LC3A in >10% of lymphoid cells (high reactivity) were associated with the hypoxia-related protein HIF1α and the enzyme of anaerobic metabolism lactate dehydrogenase LDH5 (p=0.004 and p=0.003, respectively). Such associations, however, were not a feature in DLBCLs of increased LC3A activity. CONCLUSIONS: LC3A expression in FLs is hypoxia-induced, whereas its expression in DLBCLs may be regulated by other molecular mechanisms. The current study provides a tool for further assessment of autophagic activity in translational and autophagy targeting therapy studies.

Original publication

DOI

10.1016/j.hemonc.2013.02.001

Type

Journal article

Journal

Hematol Oncol Stem Cell Ther

Publication Date

03/2013

Volume

6

Pages

20 - 25

Keywords

Antibodies, Autophagy, Humans, Hyperplasia, Hypoxia-Inducible Factor 1, alpha Subunit, Immunohistochemistry, Isoenzymes, L-Lactate Dehydrogenase, Lymph Nodes, Lymphoma, Follicular, Lymphoma, Large B-Cell, Diffuse, Microtubule-Associated Proteins, Tissue Array Analysis