Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

OBJECTIVE: The receptor-like protein-tyrosine phosphatase (PTP) CD148 and the nontransmembrane PTP1-B have been shown to be net positive regulators of Src family kinases in platelets. In the present study, we compared the relative contributions of these PTPs in platelet activation by the major glycoprotein, glycoprotein VI, α(IIb)β(3), and C-type lectin-like receptor 2 (CLEC-2). METHODS AND RESULTS: PTP-1B-deficient mouse platelets responded normally to the glycoprotein VI-specific agonist collagen-related peptide and antibody-mediated CLEC-2 activation. However, they exhibited a marginal reduction in α(IIb)β(3)-mediated Src family kinase activation and tyrosine phosphorylation. In contrast, CD148-deficient platelets exhibited a dramatic reduction in activation by glycoprotein VI and α(IIb)β(3) and a marginal reduction in response to activation by CLEC-2, which was further enhanced in the absence of PTP-1B. These defects were associated with reduced activation of Src family kinase and spleen tyrosine kinase, suggesting a causal relationship. Under arteriolar flow conditions, there was defective aggregate formation in the absence of PTP-1B and, to a greater extent, CD148 and a severe abrogation of both adhesion and aggregation in the absence of both PTPs. CONCLUSIONS: Findings from this study demonstrate that CD148 plays a dominant role in activating Src family kinases in platelets relative to PTP-1B. Both PTPs are required for optimal platelet activation and aggregate formation under high arterial shear rates.

Original publication

DOI

10.1161/ATVBAHA.112.300447

Type

Journal article

Journal

Arterioscler Thromb Vasc Biol

Publication Date

12/2012

Volume

32

Pages

2956 - 2965

Keywords

Animals, Blood Platelets, Cell Adhesion, Fibrinogen, Glycoproteins, In Vitro Techniques, Lectins, C-Type, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Animal, Platelet Activation, Platelet Aggregation, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Receptor-Like Protein Tyrosine Phosphatases, Class 3, Signal Transduction, src-Family Kinases