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Cells lacking expression of the transporter associated with antigen processing (TAP) are deficient in surface HLA class I, yet express reduced levels of HLA-A2 antigen through TAP-independent processing pathways. We have analysed the expression of HLA-A, -B and -C antigens on the 721.174 and T2 TAP-deficient mutant cell lines using a panel of monoclonal antibodies specific for the HLA antigens encoded by the genotype of these cells. Our study has shown the constitutive expression of HLA-Cw1 molecules on the cell surface of both T2 and 721.174 cells and has confirmed that HLA-A2 and HLA-B51 are expressed at low levels. Transfection of 721.174 cells with cDNAs encoding TAP1 and TAP2 proteins did not fully restore HLA class I antigen expression on these cells, which appeared to be mainly due to a deficiency in expression of the HLA-B51-associated Bw4 epitope. This suggests that additional antigen-processing genes may be required for optimal generation of HLA-B-binding peptides. Our results indicate that TAP-independent pathways of antigen-processing provide peptides for functional expression of all three classical HLA class I molecules.

Type

Journal article

Journal

Tissue Antigens

Publication Date

10/1998

Volume

52

Pages

368 - 373

Keywords

ATP-Binding Cassette Sub-Family B Member 2, ATP-Binding Cassette Transporters, ATP-Binding Cassette, Sub-Family B, Member 3, Alleles, Animals, Antibodies, Monoclonal, Cell Line, Flow Cytometry, Genotype, HLA Antigens, Humans, Major Histocompatibility Complex, Mice