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AIMS/HYPOTHESIS: Fatty acid entrapment in femoral adipose tissue has been proposed to prevent ectopic fat deposition and visceral fat accumulation, resulting in protection from insulin resistance. Our objective was to test the hypothesis of femoral, compared with abdominal, adipose tissue resistance to adrenergic stimulation in vivo as a possible mechanism. METHODS: Regional fatty acid trafficking, along with the measurement of adipose tissue blood flow (ATBF) with (133)Xe washout, was studied with the arteriovenous difference technique and stable isotope tracers in healthy volunteers. Adrenergic agonists (isoprenaline, adrenaline [epinephrine]) were infused either locally by microinfusion or systemically. Local microinfusion of adrenoceptor antagonists (propranolol, phentolamine) was used to characterise specific adrenoceptor subtype effects in vivo. RESULTS: Femoral adipose tissue NEFA release and ATBF were lower during adrenaline stimulation than in abdominal tissue (p < 0.001). Mechanistically, femoral adipose tissue displayed a dominant α-adrenergic response during adrenaline stimulation. The α-adrenoceptor blocker, phentolamine, resulted in the 'disinhibition' of the femoral ATBF response to adrenaline (p < 0.001). CONCLUSIONS/INTERPRETATION: Fatty acids, once stored in femoral adipose tissue, are not readily released upon adrenergic stimulation. Femoral adipose tissue resistance to adrenaline may contribute to the prevention of ectopic fatty acid deposition.

Original publication

DOI

10.1007/s00125-012-2676-0

Type

Journal article

Journal

Diabetologia

Publication Date

11/2012

Volume

55

Pages

3029 - 3037

Keywords

Adrenergic alpha-Antagonists, Adrenergic beta-Agonists, Adrenergic beta-Antagonists, Adult, Blood Pressure, Epinephrine, Fatty Acids, Female, Femur, Heart Rate, Humans, Intra-Abdominal Fat, Isoproterenol, Lipolysis, Male, Phentolamine, Propranolol, Sex Characteristics, Subcutaneous Fat, Vascular Resistance, Xenon Radioisotopes