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The polyubiquitin gene of the human protozoan parasite Plasmodium falciparum (PfpUB) was cloned and shown to be comprised of five tandem repeats of the ubiquitin open reading frame, present as a single copy on chromosome 12. The 1672 bp of PfpUB is interrupted at the 5' end by a single intron of 526 bp. PfpUB expression is developmentally regulated in intraerythrocytic stages with a marked increase in both steady-state transcript and polyubiquitin protein levels in late trophozoite stages. On response to heat shock, late stage parasites (late trophozoites and schizonts) have a slightly elevated PfpUB transcript level as well as readily observable increases in the amount of polyubiquitin and ubiquitin-conjugated proteins.

Type

Journal article

Journal

Mol Biochem Parasitol

Publication Date

05/01/2000

Volume

105

Pages

115 - 125

Keywords

Amino Acid Sequence, Animals, Biopolymers, Blotting, Northern, Cloning, Molecular, DNA, Protozoan, Erythrocytes, Evolution, Molecular, Gene Expression Regulation, Heat-Shock Response, Humans, Molecular Sequence Data, Open Reading Frames, Plasmodium falciparum, Polyubiquitin, Sequence Analysis, DNA, Tandem Repeat Sequences, Transcription, Genetic, Ubiquitins