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The class I CD8 positive T-cell response is involved in a number of conditions in which artificial down-regulation and control would be therapeutically beneficial. Such conditions include a number of autoimmune diseases and graft rejection in transplant patients. Although the CD8 T-cell response is dominated by the TCR-pMHC interaction, activation of T cells is in most cases also dependent on a number of associated signalling molecules. Previous work has demonstrated the ability of one such molecule (CD8) to act as an antagonist to T-cell activation if added in soluble form. Therefore, a high-affinity mutant CD8 (haCD8) has been developed with the aim of developing a therapeutic immunosuppressor. In order to fully understand the nature of the haCD8 interaction, this protein was crystallized using the sitting-drop vapour-diffusion method. Single haCD8 crystals were cryocooled and used for data collection. These crystals belonged to space group P6 422 (assumed by similarity to the wild type), with unit-cell parameters a = 101.08, c = 56.54 Å. V M calculations indicated one molecule per asymmetric unit. A 2 Å data set was collected and the structure is currently being determined using molecular replacement. © 2005 International Union of Crystallography. All rights reserved.

Original publication

DOI

10.1107/S1744309105002988

Type

Journal article

Journal

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Publication Date

01/12/2005

Volume

61

Pages

285 - 287