Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Using oligonucleotide primers that hybridize to conserved sequences in the reverse transcriptase (RT) gene, we have amplified by the polymerase chain reaction three sequence variants of HTLV-I from the genomic DNA of five patients with tropical spastic paraparesis (TSP), and a fourth sequence variant from a healthy carrier of HTLV-I. These results unequivocally identify the retrovirus associated with TSP as HTLV-I and suggest that no sequence variant is uniquely responsible for the condition. The same primers served to amplify two novel single-copy endogenous retroviral RT sequences related to the exogenous mammalian leukaemia viruses: and three KpnI (LINE1) family DNA repeats. This strategy, combining the sensitivity of PCR with cross-reactive primers, may be useful in the search for known or novel retroviruses in other diseases of possible retroviral aetiology.

Type

Journal article

Journal

EMBO J

Publication Date

20/12/1988

Volume

7

Pages

4179 - 4184

Keywords

Amino Acid Sequence, Base Sequence, DNA, Viral, DNA-Directed DNA Polymerase, Gene Amplification, Genes, Viral, Human T-lymphotropic virus 1, Humans, Molecular Sequence Data, Paraparesis, Tropical Spastic, RNA-Directed DNA Polymerase, Repetitive Sequences, Nucleic Acid, Taq Polymerase