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The alpha-glucosidase inhibitor N-butyldeoxynojirimycin (NB-DNJ) is a potent inhibitor of human immunodeficiency virus (HIV) replication and syncytium formation in vitro. However, the exact mechanism of action of NB-DNJ remains to be determined. In this study we have examined the impairment of HIV infectivity mediated by NB-DNJ. By two independent HIV entry assays [PCR-based HIV entry assay and entry of Cocal(HIV) pseudotypes], the reduction in infectivity was found to be due to an impairment of viral entry. No effect of NB-DNJ treatment was seen on the kinetics of the interaction between gp120 and CD4 (surface plasmon resonance; BIAcore) or on the binding of virus particles to H9 cells (using radiolabeled virions). We therefore conclude that a major mechanism of action of NB-DNJ as an inhibitor of HIV replication is the impairment of viral entry at the level of post-CD4 binding, due to an effect on viral envelope components.

Type

Journal article

Journal

J Virol

Publication Date

09/1995

Volume

69

Pages

5791 - 5797

Keywords

1-Deoxynojirimycin, Antiviral Agents, CD4 Antigens, Cell Line, Glycoside Hydrolase Inhibitors, HIV Envelope Protein gp120, HIV Reverse Transcriptase, HIV-1, Humans, Kinetics, RNA-Directed DNA Polymerase, Receptors, Virus, Time Factors, Virion, Virus Replication