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The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. Recently, we identified the Runx1 +23 enhancer that targets reporter gene expression to the first emerging HSCs of the mouse embryo when linked to the heterologous hsp68 promoter. Endogenous Runx1 is transcribed from 2 alternative promoters, P1 and P2. Here, we examined the in vivo cis-regulatory potential of these alternative promoters and asked whether they act with and contribute to the spatiotemporal specific expression of the Runx1 +23 enhancer. Our results firmly establish that, in contrast to zebrafish runx1, mouse Runx1 promoter sequences do not confer any hematopoietic specificity in transgenic embryos. Yet, both mouse promoters act with the +23 enhancer to drive reporter gene expression to sites of HSC emergence and colonization, in a +23-specific pattern.

Original publication

DOI

10.1182/blood-2008-12-193003

Type

Journal article

Journal

Blood

Publication Date

21/05/2009

Volume

113

Pages

5121 - 5124

Keywords

Animals, Core Binding Factor Alpha 2 Subunit, Embryo, Mammalian, Enhancer Elements, Genetic, Hematopoietic Stem Cells, Mice, Mice, Transgenic, Promoter Regions, Genetic, Species Specificity, Transcription, Genetic