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Nonviral gene therapy utilizing plasmid DNA (pDNA) complexed with cationic lipids (lipoplexes) or cationic polymers (polyplexes) has demonstrated considerable potential for the treatment of a variety of diseases. However, progress toward clinical application is often delayed by the lack of reliable and scalable mixing of components sufficient to guarantee consistent performance in vivo. Attempts to improve and standardize mixing have been limited by the sensitivity of pDNA to shear-related degradation. Here we describe a simple pneumatic mixing device that enables the rapid and reproducible production of large volumes of nonviral gene therapy formulations and demonstrate its suitability for use with shear-sensitive pDNA.

Original publication

DOI

10.2144/000113498

Type

Journal article

Journal

Biotechniques

Publication Date

09/2010

Volume

49

Pages

666 - 668

Keywords

Animals, Cations, DNA, Equipment Design, Gene Expression, Genetic Therapy, Lipids, Mice, Mice, Inbred BALB C, Plasmids