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We have used X-linked restriction fragment length polymorphism (RFLP)-methylation and gene deletion analyses to investigate the nature of the progenitor cell of origin in the myelodysplastic syndromes (MDS). Gene deletion studies were performed on the granulocyte and T-lymphocyte fractions of six women with refractory anaemia (RA) and either a partial deletion of the long arm of chromosome 5 (5q-) or monosomy 7. All six showed gene loss in the granulocyte but not the T-lymphocyte fractions, indicating monoclonality of the granulocytes but not the T-lymphocytes. In order to further investigate this finding, we subsequently performed X-RFLP-methylation studies using the probe M27 beta, and also a probe for the phosphoglycerate kinase (PGK) gene. These studies have confirmed the monoclonality of the granulocytes and the polyclonality of the T-lymphocytes in these cases. Our findings suggest that in this group of patients with MDS the T-lymphocytes were not involved in the disorder, and furthermore, in the one case where B-lymphocytes were also available, that the progenitor cell of origin was restricted to the myeloid lineage.

Type

Journal article

Journal

Br J Haematol

Publication Date

12/1991

Volume

79

Pages

550 - 555

Keywords

Anemia, Refractory, Chromosome Deletion, Chromosomes, Human, Pair 5, Chromosomes, Human, Pair 7, Clone Cells, DNA Probes, Female, Genetic Linkage, Granulocytes, Humans, Monosomy, Phosphoglycerate Kinase, Polymorphism, Restriction Fragment Length, T-Lymphocytes, X Chromosome