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The putative transcriptional corepressor ETO/MTG8 has been extensively studied due to its involvement in a chromosomal translocation causing the t(8;21) form of acute myeloid leukemia. Despite this, the role of ETO in normal physiology has remained obscure. Here we show that ETO is highly expressed in preadipocytes and acts as an inhibitor of C/EBPbeta during early adipogenesis, contributing to its characteristically delayed activation. ETO prevents both the transcriptional activation of the C/EBPalpha promoter by C/EBPbeta and its concurrent accumulation in centromeric sites during early adipogenesis. ETO expression rapidly reduces after the initiation of adipogenesis, and this is essential to the normal induction of adipogenic gene expression. These findings define, for the first time, a molecular role for ETO in normal physiology as an inhibitor of C/EBPbeta and a novel regulator of early adipogenesis.

Original publication

DOI

10.1128/MCB.24.22.9863-9872.2004

Type

Journal article

Journal

Mol Cell Biol

Publication Date

11/2004

Volume

24

Pages

9863 - 9872

Keywords

3T3-L1 Cells, Adipocytes, Adipose Tissue, Animals, CCAAT-Enhancer-Binding Protein-beta, Cell Line, DNA-Binding Proteins, Gene Expression, Humans, Lipid Metabolism, Mice, Proto-Oncogene Proteins, RUNX1 Translocation Partner 1 Protein, Recombinant Fusion Proteins, Transcription Factors, Transfection