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Monoclonal antibodies My10, BI.3C5, 12.8, and ICH3 identify a monomeric cell surface glycoprotein (HPCA-1) of 100-120 kD, which is selectively expressed on human hemopoietic progenitor cells. Other tissues are nonreactive with the exception of capillary endothelia and basement membrane in some sites. In addition, the antigen can be detected on cell lines that exhibit characteristics associated with early T cell precursors. HPCA-1 is therefore associated with myeloid, B, and T lineage precursors. Sequential immunoprecipitation and Western blotting studies demonstrate that BI.3C5, ICH3, My10, and an antibody directed against endothelial cells, 188.27, all react with the same glycoprotein species, although the epitopes involved may be distinct. The epitope recognized by BI.3C5 is sialic acid dependent, whereas that recognized by ICH3 is not. The My10 epitope has partial sensitivity to neuraminidase. Competitive/additive binding experiments suggest that these epitopes, although probably distinct, may be closely associated.


Journal article



Publication Date





417 - 426


Leukaemia Research Fund Centre, Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.


T-Lymphocytes, Hematopoietic Stem Cells, Cell Line, Tumor Cells, Cultured, Humans, Carbohydrates, Membrane Glycoproteins, Receptors, Antigen, T-Cell, Antigens, Differentiation, Antibodies, Monoclonal, Epitopes, Genes, Immunoglobulin, Tissue Distribution