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Hematopoietic stem cells (HSCs) are considered one of the most promising therapeutic targets for the treatment of various blood disorders. However, due to difficulties in establishing stable maintenance and expansion of HSCs in vitro, their insufficient supply is a major constraint to transplantation studies. To solve these problems we have developed a fully defined, all-recombinant protein-based culture system. Through this system, we have identified hemopexin (HPX) and interleukin-1α as responsible for HSC maintenance in vitro. Subsequent molecular analysis revealed that HPX reduces intracellular reactive oxygen species levels within cultured HSCs. Furthermore, bone marrow immunostaining and 3D immunohistochemistry revealed that HPX is expressed in non-myelinating Schwann cells, known HSC niche constituents. These results highlight the utility of this fully defined all-recombinant protein-based culture system for reproducible in vitro HSC culture and its potential to contribute to the identification of factors responsible for in vitro maintenance, expansion, and differentiation of stem cell populations.

Original publication

DOI

10.1016/j.stemcr.2017.01.015

Type

Journal article

Journal

Stem Cell Reports

Publication Date

14/03/2017

Volume

8

Pages

500 - 508

Keywords

BSA, FCS, all-recombinant protein-based culture system, hematopoietic stem cell, hemopexin, Animals, Blood Proteins, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cell Self Renewal, Colony-Forming Units Assay, Hematopoietic Stem Cells, Hemopexin, Interleukin-1alpha, Mice, Recombinant Proteins