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The recombinant plasmids p324, p330 and p323 carrying 5' flanking c-myc sequences linked to the reporter gene chloramphenicol acetyl-transferase (cat) were introduced into the mouse erythroleukemia cell line F412B2TK- and stable transfectants resistant to geneticin were obtained. The effects of two platinum coordination compounds, cisplatin and carboplatin, were studied using a wide range of drug concentrations. It was found that cisplatin stimulates cat gene expression with maximum effect at 5 x 10(-5) M concentration, while carboplatin at concentrations from 1 x 10(-6) to 1 x 10(-4) M did not have any effect. These results demonstrate that cisplatin stimulates transcription from the c-myc promoter in erythroleukemia cells and may support the use of carboplatin as compared to cisplatin in the treatment of cancer patients.

Original publication

DOI

10.1097/00001813-199112000-00011

Type

Journal article

Journal

Anticancer Drugs

Publication Date

12/1991

Volume

2

Pages

597 - 601

Keywords

Animals, Carboplatin, Cell Division, Chloramphenicol O-Acetyltransferase, Cisplatin, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Leukemic, Genes, myc, Kinetics, Leukemia, Erythroblastic, Acute, Mice, Plasmids, Promoter Regions, Genetic, Stimulation, Chemical, Tumor Cells, Cultured