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Three sublines have been derived from the parental line Mv1Lu by transfection with normal and mutated Ha-ras, and myc oncogenes, and subsequent cloning. All the oncogenes have increased the growth rate of the cell in vitro, increased their plating efficiency in monolayer and suspension, and reduced their serum dependence. Growth in vivo as xenografts in nude mice has also been increased. Very few tumours were generated from the parental line and those that did form did so after a prolonged lag period, while the transfected lines produced tumours with 100% efficiency, and a short lag period. In general the effects of ras transfection were more extreme, with the highest growth rates and plating efficiencies in vitro and the shortest lag period and doubling times in vivo. There was no increase in plasminogen activator activity as a result of transfection, and the invasive behaviour of the lines in organotypic culture was broadly similar.

Type

Journal article

Journal

Anticancer Res

Publication Date

05/1991

Volume

11

Pages

1343 - 1348

Keywords

Animals, Cell Line, Cell Transformation, Neoplastic, Genes, myc, Genes, ras, Lung, Mice, Mink, Neoplasm Invasiveness, Neoplasm Transplantation, Neoplasms, Experimental, Oncogenes, Plasminogen Activators, Transfection