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1. The three-dimensional structure of human lung tumour spheroids conferred a degree of resistance to the anthracyclines adriamycin, 4'-deoxydoxorubicin, daunomycin and daunomycin-low density lipoprotein complex in comparison with cells grown as a monolayer, as assessed by delayed growth and clonogenic cell survival. 2. 4'-Deoxydoxorubicin induced a longer growth delay and greater clonogenic cell kill than adriamycin in spheroids, although it was no more cytotoxic in monolayer. 3. Fluorescent microscopy demonstrated that the more lipophilic analogues partitioned into the spheroid more rapidly and to a greater degree than adriamycin. 4. The spheroid model demonstrated that penetration is an important aspect of resistance to anthracycline drugs, and this approach may represent a better in vitro system for testing lipophilic analogues of cytotoxic drugs.

Original publication

DOI

10.3109/00498258809041702

Type

Journal article

Journal

Xenobiotica

Publication Date

06/1988

Volume

18

Pages

641 - 648

Keywords

Antibiotics, Antineoplastic, Cell Aggregation, Cell Division, Cell Line, Cell Survival, Humans, Kinetics, Lung Neoplasms, Naphthacenes, Tumor Cells, Cultured, Tumor Stem Cell Assay