Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

OBJECTIVE: We assessed the causes of imbalance of oxygen transport by continuously measuring oxygen consumption (VO2) during hypothermic cardiopulmonary bypass (CPB) in pigs. METHODS: Six pigs (17.2+/-1.6 kg) underwent hypothermic (32 degrees C) CPB for 180 min with 120 min of aortic crossclamping (ACC). An AMIS 2000 mass spectrometer was adapted for the on-line measurement of VO2. Arterial lactate was measured at the beginning of CPB, the end of hypothermia, before and 10 min after ACC release, 20 min later, and at the end of CPB. RESULTS: Arterial lactate increased from 1.8+/-0.7 to 5.1+/-1.8 mmol/L during CPB. Hypothermia reduced VO2 by 0.63+/-0.29 mlmin/kg per degrees C, but lactate increased to 4.2+/-1.5 mmol/L (p <0.05). The most rapid rise of VO2 and lactate occurred during the first 10 min after ACC removal, accounting for 26% and 68%, respectively, of the total rise during rewarming. CONCLUSIONS: Inadequate tissue oxygenation persists throughout hypothermic CPB. The rise in systemic VO2 and lactate immediately after ACC release may reflect inadequate oxygen transport within the myocardium during ischemia and manifest on reperfusion. This simple technique may be used to provide important information regarding the dynamic balance of systemic and myocardial oxygen transport during ischemia-reperfusion.

Original publication

DOI

10.1191/0267659105pf823oa

Type

Journal article

Journal

Perfusion

Publication Date

09/2005

Volume

20

Pages

277 - 283

Keywords

Animals, Arteries, Cardiopulmonary Bypass, Hypothermia, Induced, Kinetics, Lactic Acid, Mass Spectrometry, Metabolism, Models, Animal, Myocardial Ischemia, Myocardium, Oxygen, Oxygen Consumption, Reperfusion, Rewarming, Swine