BSc, MSc, PhD
Senior postdoctoral researcher
My research is focused on the role of recurrent mutations observed in myelodysplastic syndrome (MDS). MDS represents a heterogeneous group of clonal hematopoietic stem cell (HSC) malignancies characterized by ineffective haematopoiesis leading to peripheral blood cytopenias, and MDS patients show increasing bone marrow blasts.
Over the last 10 years, next generation sequencing has revolutionized our understanding of the pathogenesis of this disease, establishing that MDS arises through the sequential acquisition of somatic mutations in HSCs. A large number of driver mutations have been identified in MDS, and the affected genes can be organized into a number of categories including RNA splicing factors, epigenetic regulators, cohesion components, transcription factors, DNA damage response and signal transduction.
We are using the latest technologies to investigate the role of these mutated genes in MDS. CRISPR/Cas9, a novel genome editing tool has transformed research in molecular biology and we are currently using this technique to investigate the impact of driver mutations on the MDS phenotype. Furthermore, we are using CRISPR/Cas9 together with induced pluripotent stem cells to model MDS. These studies will allow for the determination of the impact of these mutations, both individually and in combination, on the MDS phenotype and for the identification of the critical downstream targets in the main cell lineages affected in MDS.
I am currently the departmental Deputy Biological Safety Officer and also the health and safety officer of Bloodwise Molecular Haematology Unit.
SF3B1 mutations induce R-loop accumulation and DNA damage in MDS and leukemia cells with therapeutic implications.
Singh S. et al, (2020), Leukemia, 34, 2525 - 2530
Application of induced pluripotent stem cell technology for the investigation of hematological disorders.
Dolatshad H. et al, (2019), Adv Biol Regul, 71, 19 - 33
Impact of spliceosome mutations on RNA splicing in myelodysplasia: dysregulated genes/pathways and clinical associations.
Pellagatti A. et al, (2018), Blood, 132, 1225 - 1240
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins.
Naeimi Kararoudi M. et al, (2018), J Vis Exp
Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Gene Editing Technique in Xenotransplantation.
Naeimi Kararoudi M. et al, (2018), Front Immunol, 9