I joined the Milne lab in 2018 after completing my PhD in the lab of Joan Boyes at the University of Leeds in 2018, where I investigated the temporal regulation of V(D)J recombination. My current research investigates the emergence of novel enhancers in leukaemia, with a focus on MLL-AF4 Acute Lymphoblastic Leukaemia (ALL).
I use a wide variety of high-throughput techniques (ChIP-seq, low cell number ChIPMentation, ATAC-seq, RNA-seq and Capture-C ) together with machine learning and other bioinformatic methods to better understand the regulation of chromatin and gene expression.
A human genome editing-based MLL::AF4 B-cell ALL model recapitulates key cellular and molecular leukemogenic features.
Bueno C. et al, (2023), Blood
Temporal analyses reveal a pivotal role for sense and antisense enhancer RNAs in coordinate immunoglobulin lambda locus activation.
Gao Z. et al, (2023), Nucleic Acids Res
Dysregulation of chromatin via H3K27 methylation underpins differentiation arrest in Isocitrate dehydrogenase-mutant Acute Myeloid Leukaemia
Silveira DRA. et al, (2023)
MLL-AF4 cooperates with PAF1 and FACT to drive high-density enhancer interactions in leukemia.
Crump NT. et al, (2023), Nat Commun, 14
Author Correction: Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
Downes DJ. et al, (2023), Nat Protoc