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Plasmid DNA (pDNA) expression vectors are fundamental to all forms of non-viral gene transfer. In this review, we discuss principles of pDNA design and production including the impact of bacterially derived sequences on transgene expression and minicircle approaches to minimize their effects. The impact of inclusion of DNA elements such as scaffold matrix attachment regions (S/MARs), transcription factor (TF)-binding sites and tissue-specific promoters are described. The benefits of eliminating CG dinucleotides (CpGs) from the pDNA are also considered.

Original publication

DOI

10.1038/gt.2008.183

Type

Journal article

Journal

Gene Ther

Publication Date

02/2009

Volume

16

Pages

165 - 171

Keywords

Drug Design, Gene Expression Regulation, Gene Transfer Techniques, Genetic Vectors, Humans, Inverted Repeat Sequences, Plasmids, Promoter Regions, Genetic, Transgenes