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The cellular signalling process is a highly complex mechanism, involving multiple players, which together orchestrate the cell's response to environmental changes and perturbations. Given the multitude of genes that participate in the process of cellular signalling, its study in a genome-wide manner has proven challenging. Recent advances in gene editing technologies, including clustered regularly-interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) approaches, have opened new opportunities to investigate global regulatory signalling programs of cells in an unbiased manner. In this review, we focus on how the application of pooled genetic screening approaches using the CRISPR/Cas9 system has contributed to a systematic understanding of cellular signalling processes in normal and disease contexts.

Original publication

DOI

10.3390/ijms19040933

Type

Journal article

Journal

Int J Mol Sci

Publication Date

21/03/2018

Volume

19

Keywords

CRISPR/Cas9, cellular signalling, genome-wide screens, CRISPR-Cas Systems, Cells, Genetic Testing, Genome, Signal Transduction