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A recombinant plasmid carrying the long terminal repeat (LTR) of the human immunodeficiency virus 1 (HIV-1) linked to the reporter chloramphenicol acetyl transferase (CAT) gene was stably introduced into rat liver cells. The transfectant cells expressed CAT activity from the HIV LTR. The response to doxorubicin was studied and it was found that at the optimum concentration of 20 micrograms/ml doxorubicin, the expression of CAT from the HIV LTR was stimulated by 65-fold. Our results suggest caution against therapy including doxorubicin in the treatment of AIDS patients.

Original publication

DOI

10.1016/0304-3835(91)90094-x

Type

Journal article

Journal

Cancer Lett

Publication Date

02/1991

Volume

56

Pages

181 - 185

Keywords

Animals, Azacitidine, Cell Division, Cell Line, Chloramphenicol O-Acetyltransferase, Doxorubicin, HIV Long Terminal Repeat, Kinetics, Liver, Plasmids, Rats, Recombinant Fusion Proteins, Transcription, Genetic, Transfection