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Glucose clamping has become a widely used test for assessing insulin sensitivity and beta-cell function. We present a new method of achieving a reliable and unbiased clamp using an iterative computer program. After initial parameter estimation, the program uses no fixed algorithm or physiological preconceptions but makes predictions according to previous glycemic responses to infusion rates. The program can be used for human or animal studies and for euglycemic or hyperglycemic clamps. The program has flexible data entry, graphic display, running statistics of mean infusion data, and mean glucose levels and their standard deviations. These form part of the criteria for the assessment of glucose clamping, which includes the sampling interval, the duration of the clamp, the achieved glucose concentration (together with its coefficient of variation), and the percent change of glucose infused at the end of the clamp. Data for 151 consecutive clamp studies were analyzed. Median coefficient of variation of glucose values at the end of the clamp was 2%.

Original publication

DOI

10.2337/diacare.12.2.156

Type

Journal article

Journal

Diabetes Care

Publication Date

02/1989

Volume

12

Pages

156 - 159

Keywords

Algorithms, Diabetes Mellitus, Glucose Clamp Technique, Humans, Insulin Infusion Systems, Male, Software