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A survey of the gamma-globin gene region of over 1000 normal individuals revealed a novel 2.5 kb deletion which removes the 5' end of the A gamma-globin gene. Unusually, this deletion in the beta-globin gene cluster is not associated with increased fetal haemoglobin production. Sequence analysis of the deletion endpoints revealed no significant homology at the breakpoint and failed to support a role for a proposed recombination hotspot in IVS-2 in the generation of this illegitimate recombination event. The existence of small "silent" deletions in the beta-globin gene cluster emphasizes the importance of deletion size in altering expression of the fetal globin genes.

Original publication

DOI

10.1093/nar/14.12.4743

Type

Journal article

Journal

Nucleic Acids Res

Publication Date

25/06/1986

Volume

14

Pages

4743 - 4750

Keywords

Base Sequence, Chromosome Deletion, Cloning, Molecular, DNA Restriction Enzymes, Fetal Hemoglobin, Gene Expression Regulation, Globins, Humans, Melanesia