Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-α (PDGFRα) expression. SP status predicts clonogenicity and cardiogenic gene expression (Gata4/6, Hand2 and Tbx5/20), properties segregating more specifically to PDGFRα(+) cells. Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. PDGFRα(-) cells are characterized by Kdr/Flk1, Cdh5, CD31 and lack of clonogenicity. PDGFRα(+)/CD31(-) cells derive from cells formerly expressing Mesp1, Nkx2-5, Isl1, Gata5 and Wt1, distinct from PDGFRα(-)/CD31(+) cells (Gata5 low; Flk1 and Tie2 high). Thus, PDGFRα demarcates the clonogenic cardiogenic Sca1(+) stem/progenitor cell.

Original publication

DOI

10.1038/ncomms7930

Type

Journal article

Journal

Nat Commun

Publication Date

18/05/2015

Volume

6

Keywords

Animals, Ataxin-1, Basic Helix-Loop-Helix Transcription Factors, Cell Differentiation, Cell Lineage, Endothelial Cells, Female, GATA4 Transcription Factor, Gene Expression Regulation, Male, Mice, Mice, Inbred C57BL, Myocardium, Myocytes, Cardiac, Myocytes, Smooth Muscle, Phenotype, Platelet Endothelial Cell Adhesion Molecule-1, Principal Component Analysis, Receptor, Platelet-Derived Growth Factor alpha, Regeneration, Stem Cells, T-Box Domain Proteins