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BACKGROUND: The association of functionality and phenotype of follicular helper T cells (Tfh) with dengue virus (DENV) specific antibody responses and clinical disease severity has not been well studied. METHODS: We investigated the phenotype and functionality of Tfh cells and plasmablasts in adult patients (DF = 18, DHF = 22) with acute dengue (day 4 to 8 since onset of fever) of varying severity using multiparametric flowcytometry. The properties of Tfh cells were correlated with viraemia, disease severity, plasmablast responses and DENV-specific serum antibody responses. We further evaluated the kinetics of neutralizing antibodies (Neut50) throughout the course of illness in order to evaluate their association with clinical disease severity and viraemia. RESULTS: Tfh cells (especially those producing IL-21 and co-expressing PD-1 and ICOS) were found to be significantly expanded (p 

Original publication

DOI

10.1186/s12929-020-00641-2

Type

Journal article

Journal

J Biomed Sci

Publication Date

08/04/2020

Volume

27

Keywords

Follicular helper T cells, IL-21, NS1 antibodies, Neutralizing antibodies, Plasmablasts, Viral loads, Acute Disease, Adult, Antibodies, Neutralizing, Antibodies, Viral, Dengue, Dengue Virus, Glycoproteins, Humans, Middle Aged, Phenotype, T-Lymphocytes, Helper-Inducer, Viral Nonstructural Proteins, Young Adult