Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Five groups of NMRI mice were fed ethanol or sucrose in a nutritionally adequate liquid diet for 9 days. The dietary fat consisted of olive oil with the fatty acid composition 18:1 77%, 18:2 10%, 18:0 and 16:0 12%. The ethanol treated groups received 5% w/v ethanol (E) or isocaloric sucrose (S). Two groups (S- and E-) received the diet without supplement. In two groups (S+ and E+) 7% of the fat was exchanged for arachidonic acid (20:4). In a fifth group (IE+) treated with ethanol and arachidonic acid the diet also contained indomethacin (10 mg/l). The mean intake of ethanol was about 20 g/kg/day. After 9 days animals were killed and liver lipids analyzed after Folch extraction. The post mortem accumulation of prostaglandin E2 in the kidney was measured by GC-MS. Dietary 20:4 was found to protect mice against fatty liver caused both by a high fat diet alone and in combination with ethanol. The liver triglycerides were 30.7 +/- 4.3 (S-), 46.1 +/- 6.9 (E-), 6.8 +/- 0.4 (S+) and 19.4 +/- 1.8 (E+). Prostaglandin levels in the kidney were depressed by ethanol treatment. Indomethacin gave variable degrees of PG synthesis inhibition. The degree of liver triglyceride accumulation in the IE+ group was inversely proportional to the degree of PG synthesis. The data suggest a role for liver 20:4 cyclooxygenase metabolites in fatty liver caused by high fat diets and ethanol.


Journal article


Acta Pharmacol Toxicol (Copenh)

Publication Date





95 - 99


Animals, Arachidonic Acid, Arachidonic Acids, Dietary Fats, Ethanol, Fatty Liver, Kidney, Liver, Male, Mice, Phospholipids, Prostaglandins F, Triglycerides