Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Testisin is a recently identified human serine protease expressed by premeiotic testicular germ cells and is a candidate tumor suppressor for testicular cancer. Here, we report the characterization of the gene encoding testisin, designated PRSS21, and its localization on the short arm of human chromosome 16 (16p13.3) between the microsatellite marker D16S246 and the radiation hybrid breakpoint CY23HA. We have further refined the localization to cosmid 406D6 in this interval and have established that the gene is approximately 4. 5 kb in length, and contains six exons and five intervening introns. The structure of PRSS21 is very similar to the human prostasin gene (PRSS8) which maps nearby on 16p11.2, suggesting that these genes may have evolved through gene duplication. Sequence analysis showed that the two known isoforms of testisin are generated by alternative pre-mRNA splicing. A major transcription initiation site was identified 97 nucleotides upstream of the testisin translation start and conforms to a consensus initiator element. The region surrounding the transcription initiation site lacks a TATA consensus sequence, but contains a CCAAT sequence and includes a CpG island. The 5'-flanking region contains several consensus response elements including Sp1, AP1 and several testis-specific elements. Analysis of testisin gene expression in tumor cell lines shows that testisin is not expressed in testicular tumor cells but is aberrantly expressed in some tumor cell lines of non-testis origin. These data provide the basis for identifying potential genetic alterations of PRSS21 that may underlie both testicular abnormalities and tumorigenesis.

Original publication




Journal article


Biochimica et biophysica acta

Publication Date





63 - 71


Cellular Oncology Laboratory, The Queensland Institue of Medical Research, Brisbane, Queensland 4029, Australia.


Tumor Cells, Cultured, Chromosomes, Human, Pair 16, Humans, Serine Endopeptidases, Membrane Proteins, Protein Isoforms, RNA Precursors, DNA, DNA, Complementary, Chromosome Mapping, Transfection, Transcription, Genetic, Gene Expression Regulation, Enzymologic, Alternative Splicing, Amino Acid Sequence, Base Sequence, Genes, Regulator, Genetic Vectors, Cosmids, Genome, Human, Molecular Sequence Data, Male, Promoter Regions, Genetic, GPI-Linked Proteins