The effect of doxorubicin, daunorubicin and 4'-epidoxorubicin on the exogenous c-myc promoter in mouse erythroleukemia cells.
Eliopoulos A., Kerr DJ., Spandidos DA.
The recombinant plasmids p324, p330 and p323 carrying the aminoglycoside phosphotransferase (aph) gene and 5' flanking c-myc sequences linked to the reporter gene chloramphenicol acetyl-transferase (cat) were introduced into the mouse erythroleukemia cell line F412B2TK- and stable transfectants resistant to geneticin were obtained. The effects of three anthracycline compounds, doxorubicin, daunorubicin and 4'-epidoxorubicin, were studied using a large range of drug concentrations. It was found that 4'-epidoxorubicin and daunorubicin stimulate cat-gene expression with maximum effect at a concentration of 1 micrograms/ml, while doxorubicin enhances transcription from c-myc promoter at 5 micrograms/ml.