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The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3.5 A and have the orthorhombic space group P2(1)2(1)2 or P2(1)2(1)2(1). The unit cell has dimensions a = 317 A, b = 161 A and c = 41.8 A and the asymmetric unit consists of two CD4:Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis.

Original publication

DOI

10.1016/S0022-2836(05)80116-0

Type

Journal article

Journal

J Mol Biol

Publication Date

05/05/1990

Volume

213

Pages

7 - 10

Keywords

Animals, Antibodies, Monoclonal, CD4 Antigens, Crystallization, Immunoglobulin Fab Fragments, Rats, Recombinant Proteins, Solubility, X-Ray Diffraction