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MOTIVATION: The accuracy of reference genomes is important for downstream analysis but a low error rate requires expensive manual interrogation of the sequence. Here, we describe a novel algorithm (Iterative Correction of Reference Nucleotides) that iteratively aligns deep coverage of short sequencing reads to correct errors in reference genome sequences and evaluate their accuracy. RESULTS: Using Plasmodium falciparum (81% A + T content) as an extreme example, we show that the algorithm is highly accurate and corrects over 2000 errors in the reference sequence. We give examples of its application to numerous other eukaryotic and prokaryotic genomes and suggest additional applications. AVAILABILITY: The software is available at http://icorn.sourceforge.net

Original publication

DOI

10.1093/bioinformatics/btq269

Type

Journal article

Journal

Bioinformatics

Publication Date

15/07/2010

Volume

26

Pages

1704 - 1707

Keywords

Algorithms, Base Sequence, Genome, Protozoan, Genomics, Nucleotides, Plasmodium falciparum, Sequence Alignment, Sequence Analysis, DNA, Software